The siRNA suppressor RTL1 is redox-regulated through glutathionylation of a conserved cysteine in the double-stranded-RNA-binding domain.
Identifieur interne : 000299 ( Main/Exploration ); précédent : 000298; suivant : 000300The siRNA suppressor RTL1 is redox-regulated through glutathionylation of a conserved cysteine in the double-stranded-RNA-binding domain.
Auteurs : Cyril Charbonnel [France] ; Adnan K. Niazi [France] ; Emilie Elvira-Matelot [France] ; Elzbieta Nowak [Pologne] ; Matthias Zytnicki [France] ; Anne De Bures [France] ; Edouard Jobet [France] ; Alisson Opsomer [France] ; Nahid Shamandi [France] ; Marcin Nowotny [Pologne] ; Christine Carapito [France] ; Jean-Philippe Reichheld [France] ; Hervé Vaucheret [France] ; Julio Sáez-Vásquez [France]Source :
- Nucleic acids research [ 1362-4962 ] ; 2017.
Descripteurs français
- KwdFr :
- ARN des plantes (composition chimique), ARN des plantes (génétique), ARN des plantes (métabolisme), ARN double brin (composition chimique), ARN double brin (génétique), ARN double brin (métabolisme), Arabidopsis (génétique), Arabidopsis (métabolisme), Clivage de l'ARN (MeSH), Conformation d'acide nucléique (MeSH), Cystéine (génétique), Cystéine (métabolisme), Domaines protéiques (MeSH), Glutathion (métabolisme), Modèles moléculaires (MeSH), Motifs de liaison à l'ARN (génétique), Oxydoréduction (MeSH), Petit ARN interférent (génétique), Petit ARN interférent (métabolisme), Protéines d'Arabidopsis (composition chimique), Protéines d'Arabidopsis (génétique), Protéines d'Arabidopsis (métabolisme), Protéines de répression (composition chimique), Protéines de répression (génétique), Protéines de répression (métabolisme), Ribonuclease III (génétique), Ribonuclease III (métabolisme), Régions 3' non traduites (génétique), Similitude de séquences d'acides nucléiques (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH).
- MESH :
- composition chimique : ARN des plantes, ARN double brin, Protéines d'Arabidopsis, Protéines de répression.
- génétique : ARN des plantes, ARN double brin, Arabidopsis, Cystéine, Motifs de liaison à l'ARN, Petit ARN interférent, Protéines d'Arabidopsis, Protéines de répression, Ribonuclease III, Régions 3' non traduites.
- métabolisme : ARN des plantes, ARN double brin, Arabidopsis, Cystéine, Glutathion, Petit ARN interférent, Protéines d'Arabidopsis, Protéines de répression, Ribonuclease III.
- Clivage de l'ARN, Conformation d'acide nucléique, Domaines protéiques, Modèles moléculaires, Oxydoréduction, Similitude de séquences d'acides nucléiques, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- 3' Untranslated Regions (genetics), Amino Acid Sequence (MeSH), Arabidopsis (genetics), Arabidopsis (metabolism), Arabidopsis Proteins (chemistry), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Base Sequence (MeSH), Cysteine (genetics), Cysteine (metabolism), Glutathione (metabolism), Models, Molecular (MeSH), Nucleic Acid Conformation (MeSH), Oxidation-Reduction (MeSH), Protein Domains (MeSH), RNA Cleavage (MeSH), RNA, Double-Stranded (chemistry), RNA, Double-Stranded (genetics), RNA, Double-Stranded (metabolism), RNA, Plant (chemistry), RNA, Plant (genetics), RNA, Plant (metabolism), RNA, Small Interfering (genetics), RNA, Small Interfering (metabolism), RNA-Binding Motifs (genetics), Repressor Proteins (chemistry), Repressor Proteins (genetics), Repressor Proteins (metabolism), Ribonuclease III (genetics), Ribonuclease III (metabolism), Sequence Homology, Nucleic Acid (MeSH).
- MESH :
- chemical , chemistry : Arabidopsis Proteins, RNA, Double-Stranded, RNA, Plant, Repressor Proteins.
- chemical , genetics : 3' Untranslated Regions, Arabidopsis Proteins, Cysteine, RNA, Double-Stranded, RNA, Plant, RNA, Small Interfering, Repressor Proteins, Ribonuclease III.
- genetics : Arabidopsis, RNA-Binding Motifs.
- metabolism : Arabidopsis, Arabidopsis Proteins, Cysteine, Glutathione, RNA, Double-Stranded, RNA, Plant, RNA, Small Interfering, Repressor Proteins, Ribonuclease III.
- Amino Acid Sequence, Base Sequence, Models, Molecular, Nucleic Acid Conformation, Oxidation-Reduction, Protein Domains, RNA Cleavage, Sequence Homology, Nucleic Acid.
Abstract
RNase III enzymes cleave double stranded (ds)RNA. This is an essential step for regulating the processing of mRNA, rRNA, snoRNA and other small RNAs, including siRNA and miRNA. Arabidopsis thaliana encodes nine RNase III: four DICER-LIKE (DCL) and five RNASE THREE LIKE (RTL). To better understand the molecular functions of RNase III in plants we developed a biochemical assay using RTL1 as a model. We show that RTL1 does not degrade dsRNA randomly, but recognizes specific duplex sequences to direct accurate cleavage. Furthermore, we demonstrate that RNase III and dsRNA binding domains (dsRBD) are both required for dsRNA cleavage. Interestingly, the four DCL and the three RTL that carry dsRBD share a conserved cysteine (C230 in Arabidopsis RTL1) in their dsRBD. C230 is essential for RTL1 and DCL1 activities and is subjected to post-transcriptional modification. Indeed, under oxidizing conditions, glutathionylation of C230 inhibits RTL1 cleavage activity in a reversible manner involving glutaredoxins. We conclude that the redox state of the dsRBD ensures a fine-tune regulation of dsRNA processing by plant RNase III.
DOI: 10.1093/nar/gkx820
PubMed: 28981840
PubMed Central: PMC5714217
Affiliations:
- France, Pologne
- Alsace (région administrative), Grand Est, Languedoc-Roussillon, Midi-Pyrénées, Occitanie (région administrative), Île-de-France
- Castanet-Tolosan, Orsay, Perpignan, Strasbourg, Versailles
- Université Paris-Sud
Links toward previous steps (curation, corpus...)
Le document en format XML
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Warsaw</wicri:regionArea><wicri:noRegion> 02-109 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Carapito, Christine" sort="Carapito, Christine" uniqKey="Carapito C" first="Christine" last="Carapito">Christine Carapito</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Spectrométrie de Masse BioOrganique,Université de Strasbourg, CNRS, IPHC UMR 7178, 67037 Strasbourg, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Spectrométrie de Masse BioOrganique,Université de Strasbourg, CNRS, IPHC UMR 7178, 67037 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Reichheld, Jean Philippe" sort="Reichheld, Jean Philippe" uniqKey="Reichheld J" first="Jean-Philippe" last="Reichheld">Jean-Philippe Reichheld</name><affiliation wicri:level="3"><nlm:affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author><author><name sortKey="Vaucheret, Herve" sort="Vaucheret, Herve" uniqKey="Vaucheret H" first="Hervé" last="Vaucheret">Hervé Vaucheret</name><affiliation wicri:level="3"><nlm:affiliation>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Saez Vasquez, Julio" sort="Saez Vasquez, Julio" uniqKey="Saez Vasquez J" first="Julio" last="Sáez-Vásquez">Julio Sáez-Vásquez</name><affiliation wicri:level="3"><nlm:affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28981840</idno><idno type="pmid">28981840</idno><idno type="doi">10.1093/nar/gkx820</idno><idno type="pmc">PMC5714217</idno><idno type="wicri:Area/Main/Corpus">000306</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000306</idno><idno type="wicri:Area/Main/Curation">000306</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000306</idno><idno type="wicri:Area/Main/Exploration">000306</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The siRNA suppressor RTL1 is redox-regulated through glutathionylation of a conserved cysteine in the double-stranded-RNA-binding domain.</title><author><name sortKey="Charbonnel, Cyril" sort="Charbonnel, Cyril" uniqKey="Charbonnel C" first="Cyril" last="Charbonnel">Cyril Charbonnel</name><affiliation wicri:level="3"><nlm:affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author><author><name sortKey="Niazi, Adnan K" sort="Niazi, Adnan K" uniqKey="Niazi A" first="Adnan K" last="Niazi">Adnan K. Niazi</name><affiliation wicri:level="3"><nlm:affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author><author><name sortKey="Elvira Matelot, Emilie" sort="Elvira Matelot, Emilie" uniqKey="Elvira Matelot E" first="Emilie" last="Elvira-Matelot">Emilie Elvira-Matelot</name><affiliation wicri:level="3"><nlm:affiliation>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Nowak, Elzbieta" sort="Nowak, Elzbieta" uniqKey="Nowak E" first="Elzbieta" last="Nowak">Elzbieta Nowak</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Protein Structure, International Institute of Molecular and Cell Biology, 02-109 Warsaw, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Laboratory of Protein Structure, International Institute of Molecular and Cell Biology, 02-109 Warsaw</wicri:regionArea><wicri:noRegion> 02-109 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Zytnicki, Matthias" sort="Zytnicki, Matthias" uniqKey="Zytnicki M" first="Matthias" last="Zytnicki">Matthias Zytnicki</name><affiliation wicri:level="3"><nlm:affiliation>UMIAT, INRA UR-875, F-31326 Castanet-Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMIAT, INRA UR-875, F-31326 Castanet-Tolosan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet-Tolosan</settlement></placeName></affiliation></author><author><name sortKey="De Bures, Anne" sort="De Bures, Anne" uniqKey="De Bures A" first="Anne" last="De Bures">Anne De Bures</name><affiliation wicri:level="3"><nlm:affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author><author><name sortKey="Jobet, Edouard" sort="Jobet, Edouard" uniqKey="Jobet E" first="Edouard" last="Jobet">Edouard Jobet</name><affiliation wicri:level="3"><nlm:affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author><author><name sortKey="Opsomer, Alisson" sort="Opsomer, Alisson" uniqKey="Opsomer A" first="Alisson" last="Opsomer">Alisson Opsomer</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Spectrométrie de Masse BioOrganique,Université de Strasbourg, CNRS, IPHC UMR 7178, 67037 Strasbourg, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Spectrométrie de Masse BioOrganique,Université de Strasbourg, CNRS, IPHC UMR 7178, 67037 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Shamandi, Nahid" sort="Shamandi, Nahid" uniqKey="Shamandi N" first="Nahid" last="Shamandi">Nahid Shamandi</name><affiliation wicri:level="3"><nlm:affiliation>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Université Paris-Sud, Université Paris-Saclay, 91405 Orsay, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université Paris-Sud, Université Paris-Saclay, 91405 Orsay</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Orsay</settlement></placeName><orgName type="university">Université Paris-Sud</orgName></affiliation></author><author><name sortKey="Nowotny, Marcin" sort="Nowotny, Marcin" uniqKey="Nowotny M" first="Marcin" last="Nowotny">Marcin Nowotny</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Protein Structure, International Institute of Molecular and Cell Biology, 02-109 Warsaw, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Laboratory of Protein Structure, International Institute of Molecular and Cell Biology, 02-109 Warsaw</wicri:regionArea><wicri:noRegion> 02-109 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Carapito, Christine" sort="Carapito, Christine" uniqKey="Carapito C" first="Christine" last="Carapito">Christine Carapito</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Spectrométrie de Masse BioOrganique,Université de Strasbourg, CNRS, IPHC UMR 7178, 67037 Strasbourg, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Spectrométrie de Masse BioOrganique,Université de Strasbourg, CNRS, IPHC UMR 7178, 67037 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Reichheld, Jean Philippe" sort="Reichheld, Jean Philippe" uniqKey="Reichheld J" first="Jean-Philippe" last="Reichheld">Jean-Philippe Reichheld</name><affiliation wicri:level="3"><nlm:affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author><author><name sortKey="Vaucheret, Herve" sort="Vaucheret, Herve" uniqKey="Vaucheret H" first="Hervé" last="Vaucheret">Hervé Vaucheret</name><affiliation wicri:level="3"><nlm:affiliation>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Saez Vasquez, Julio" sort="Saez Vasquez, Julio" uniqKey="Saez Vasquez J" first="Julio" last="Sáez-Vásquez">Julio Sáez-Vásquez</name><affiliation wicri:level="3"><nlm:affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author></analytic><series><title level="j">Nucleic acids research</title><idno type="eISSN">1362-4962</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3' Untranslated Regions (genetics)</term><term>Amino Acid Sequence (MeSH)</term><term>Arabidopsis (genetics)</term><term>Arabidopsis (metabolism)</term><term>Arabidopsis Proteins (chemistry)</term><term>Arabidopsis Proteins (genetics)</term><term>Arabidopsis Proteins (metabolism)</term><term>Base Sequence (MeSH)</term><term>Cysteine (genetics)</term><term>Cysteine (metabolism)</term><term>Glutathione (metabolism)</term><term>Models, Molecular (MeSH)</term><term>Nucleic Acid Conformation (MeSH)</term><term>Oxidation-Reduction (MeSH)</term><term>Protein Domains (MeSH)</term><term>RNA Cleavage (MeSH)</term><term>RNA, Double-Stranded (chemistry)</term><term>RNA, Double-Stranded (genetics)</term><term>RNA, Double-Stranded (metabolism)</term><term>RNA, Plant (chemistry)</term><term>RNA, Plant (genetics)</term><term>RNA, Plant (metabolism)</term><term>RNA, Small Interfering (genetics)</term><term>RNA, Small Interfering (metabolism)</term><term>RNA-Binding Motifs (genetics)</term><term>Repressor Proteins (chemistry)</term><term>Repressor Proteins (genetics)</term><term>Repressor Proteins (metabolism)</term><term>Ribonuclease III (genetics)</term><term>Ribonuclease III (metabolism)</term><term>Sequence Homology, Nucleic Acid (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (composition chimique)</term><term>ARN des plantes (génétique)</term><term>ARN des plantes (métabolisme)</term><term>ARN double brin (composition chimique)</term><term>ARN double brin (génétique)</term><term>ARN double brin (métabolisme)</term><term>Arabidopsis (génétique)</term><term>Arabidopsis (métabolisme)</term><term>Clivage de l'ARN (MeSH)</term><term>Conformation d'acide nucléique (MeSH)</term><term>Cystéine (génétique)</term><term>Cystéine (métabolisme)</term><term>Domaines protéiques (MeSH)</term><term>Glutathion (métabolisme)</term><term>Modèles moléculaires (MeSH)</term><term>Motifs de liaison à l'ARN (génétique)</term><term>Oxydoréduction (MeSH)</term><term>Petit ARN interférent (génétique)</term><term>Petit ARN interférent (métabolisme)</term><term>Protéines d'Arabidopsis (composition chimique)</term><term>Protéines d'Arabidopsis (génétique)</term><term>Protéines d'Arabidopsis (métabolisme)</term><term>Protéines de répression (composition chimique)</term><term>Protéines de répression (génétique)</term><term>Protéines de répression (métabolisme)</term><term>Ribonuclease III (génétique)</term><term>Ribonuclease III (métabolisme)</term><term>Régions 3' non traduites (génétique)</term><term>Similitude de séquences d'acides nucléiques (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Arabidopsis Proteins</term><term>RNA, Double-Stranded</term><term>RNA, Plant</term><term>Repressor Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>3' Untranslated Regions</term><term>Arabidopsis Proteins</term><term>Cysteine</term><term>RNA, Double-Stranded</term><term>RNA, Plant</term><term>RNA, Small Interfering</term><term>Repressor Proteins</term><term>Ribonuclease III</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ARN des plantes</term><term>ARN double brin</term><term>Protéines d'Arabidopsis</term><term>Protéines de répression</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>RNA-Binding Motifs</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term><term>ARN double brin</term><term>Arabidopsis</term><term>Cystéine</term><term>Motifs de liaison à l'ARN</term><term>Petit ARN interférent</term><term>Protéines d'Arabidopsis</term><term>Protéines de répression</term><term>Ribonuclease III</term><term>Régions 3' non traduites</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Arabidopsis Proteins</term><term>Cysteine</term><term>Glutathione</term><term>RNA, Double-Stranded</term><term>RNA, Plant</term><term>RNA, Small Interfering</term><term>Repressor Proteins</term><term>Ribonuclease III</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN des plantes</term><term>ARN double brin</term><term>Arabidopsis</term><term>Cystéine</term><term>Glutathion</term><term>Petit ARN interférent</term><term>Protéines d'Arabidopsis</term><term>Protéines de répression</term><term>Ribonuclease III</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Models, Molecular</term><term>Nucleic Acid Conformation</term><term>Oxidation-Reduction</term><term>Protein Domains</term><term>RNA Cleavage</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clivage de l'ARN</term><term>Conformation d'acide nucléique</term><term>Domaines protéiques</term><term>Modèles moléculaires</term><term>Oxydoréduction</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">RNase III enzymes cleave double stranded (ds)RNA. This is an essential step for regulating the processing of mRNA, rRNA, snoRNA and other small RNAs, including siRNA and miRNA. Arabidopsis thaliana encodes nine RNase III: four DICER-LIKE (DCL) and five RNASE THREE LIKE (RTL). To better understand the molecular functions of RNase III in plants we developed a biochemical assay using RTL1 as a model. We show that RTL1 does not degrade dsRNA randomly, but recognizes specific duplex sequences to direct accurate cleavage. Furthermore, we demonstrate that RNase III and dsRNA binding domains (dsRBD) are both required for dsRNA cleavage. Interestingly, the four DCL and the three RTL that carry dsRBD share a conserved cysteine (C230 in Arabidopsis RTL1) in their dsRBD. C230 is essential for RTL1 and DCL1 activities and is subjected to post-transcriptional modification. Indeed, under oxidizing conditions, glutathionylation of C230 inhibits RTL1 cleavage activity in a reversible manner involving glutaredoxins. We conclude that the redox state of the dsRBD ensures a fine-tune regulation of dsRNA processing by plant RNase III.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28981840</PMID><DateCompleted><Year>2017</Year><Month>12</Month><Day>12</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1362-4962</ISSN><JournalIssue CitedMedium="Internet"><Volume>45</Volume><Issue>20</Issue><PubDate><Year>2017</Year><Month>Nov</Month><Day>16</Day></PubDate></JournalIssue><Title>Nucleic acids research</Title><ISOAbbreviation>Nucleic Acids Res</ISOAbbreviation></Journal><ArticleTitle>The siRNA suppressor RTL1 is redox-regulated through glutathionylation of a conserved cysteine in the double-stranded-RNA-binding domain.</ArticleTitle><Pagination><MedlinePgn>11891-11907</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/nar/gkx820</ELocationID><Abstract><AbstractText>RNase III enzymes cleave double stranded (ds)RNA. This is an essential step for regulating the processing of mRNA, rRNA, snoRNA and other small RNAs, including siRNA and miRNA. Arabidopsis thaliana encodes nine RNase III: four DICER-LIKE (DCL) and five RNASE THREE LIKE (RTL). To better understand the molecular functions of RNase III in plants we developed a biochemical assay using RTL1 as a model. We show that RTL1 does not degrade dsRNA randomly, but recognizes specific duplex sequences to direct accurate cleavage. Furthermore, we demonstrate that RNase III and dsRNA binding domains (dsRBD) are both required for dsRNA cleavage. Interestingly, the four DCL and the three RTL that carry dsRBD share a conserved cysteine (C230 in Arabidopsis RTL1) in their dsRBD. C230 is essential for RTL1 and DCL1 activities and is subjected to post-transcriptional modification. Indeed, under oxidizing conditions, glutathionylation of C230 inhibits RTL1 cleavage activity in a reversible manner involving glutaredoxins. We conclude that the redox state of the dsRBD ensures a fine-tune regulation of dsRNA processing by plant RNase III.</AbstractText><CopyrightInformation>© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Charbonnel</LastName><ForeName>Cyril</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Niazi</LastName><ForeName>Adnan K</ForeName><Initials>AK</Initials><AffiliationInfo><Affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Elvira-Matelot</LastName><ForeName>Emilie</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nowak</LastName><ForeName>Elzbieta</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Laboratory of Protein Structure, International Institute of Molecular and Cell Biology, 02-109 Warsaw, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zytnicki</LastName><ForeName>Matthias</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>UMIAT, INRA UR-875, F-31326 Castanet-Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Bures</LastName><ForeName>Anne</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jobet</LastName><ForeName>Edouard</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Opsomer</LastName><ForeName>Alisson</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratoire de Spectrométrie de Masse BioOrganique,Université de Strasbourg, CNRS, IPHC UMR 7178, 67037 Strasbourg, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shamandi</LastName><ForeName>Nahid</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université Paris-Sud, Université Paris-Saclay, 91405 Orsay, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nowotny</LastName><ForeName>Marcin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Protein Structure, International Institute of Molecular and Cell Biology, 02-109 Warsaw, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carapito</LastName><ForeName>Christine</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Laboratoire de Spectrométrie de Masse BioOrganique,Université de Strasbourg, CNRS, IPHC UMR 7178, 67037 Strasbourg, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reichheld</LastName><ForeName>Jean-Philippe</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vaucheret</LastName><ForeName>Hervé</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, UMR1318 INRA AgroParisTech CNRS, Université Paris-Saclay, 78000 Versailles, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sáez-Vásquez</LastName><ForeName>Julio</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>CNRS, Laboratoire Génome et Développement des Plantes, UMR 5096, 66860 Perpignan, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR 5096, F-66860 Perpignan, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nucleic Acids Res</MedlineTA><NlmUniqueID>0411011</NlmUniqueID><ISSNLinking>0305-1048</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020413">3' Untranslated Regions</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012330">RNA, 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